RT-PCR analysis for PLAC1 and DPPA2 expression in a normal tissue panel. (A) PLAC1 mRNA expression yielded a 328 bp product. (B) DPPA2 mRNA expression yielded a 430 bp product. (C) GAPDH (glyceraldehyde-3-phosphate dehydrogenase) served as an endogenous control and yielded a 204 bp product. Lanes: M, marker; 1, blank; 2, placenta; 3, adrenal gland; 4, bone marrow; 5, brain; 6, cerebellum; 7, fetal liver; 8, fetal brain; 9, heart; 10, placenta; 11, prostate; 12, testis.