CT Gene

Primers

Amplified Fragment
(bp)

SYCP1

F: 5'-GAGCTGCTCATTCGTTTGTG-3'
R: 5'-TGAAAGCTTGTTGCAGTGTG-3'

425

SLCO6A1

F: 5'-TGGCAACTACACTTGCAGGA-3'
R: 5'-CGGGTCTGGCATCAATAAAA-3'

447

NY-ESO-1

F: 5'-GCTTCAGGGCTGAATGGAT-3'
R: 5'-AAAAACACGGGCAGAAAGC-3'

307

MAGEA1

F: 5'-GCTGGAACCCTCACTGGGTTGCC-3'
R: 5'-CGGCCGAAGGAACCTGACCCAG-3'

421

MAGEA3/6

F: 5'-GAAGCCGGCCCAGGCTCG-3'
R: 5'-GGAGTCCTCATAGGATTGGCT-3'

423

MAGEA4

F: 5'-GAGCAGACAGGCCAACCG-3'
R: 5'-AAGGACTCTGCGTCAGGC-3'

445

MAGEC1

F: 5'-GACGAGGATCGTCTCAGGTCAGC-3'
R: 5'-ACATCCTCACCCTCAGGAGGG-3'

623

MAGEC2

F: 5'-GGGAATCTGACGGATCGGA-3'
R: 5'-GGAATGGAACGCCTGGAAC-3'

356 (MAGEC2)
430 (MAGEC2M)

GAGE1

CT4.1, 4.2 and 4.8:
F1: 5'-GACCAAGACGCTACGTAG-3'
R1: 5'-CCATCAGGACCATCTTCA-3'
CT4.3, 4.4, 4.5, 4.6, 4.7
F2: 5'-GACCAAGGCGCTATGTAC-3'
R2: 5'-CCATCAGGACCATCTTCA-3'

243