NaPi2b/MX35 antigen biochemical characterization. Monoclonal antibody MX35 immune precipitations from metabolically-labeled SK-RC-18 cell lysates separated by SDS-PAGE under reducing conditions and visualized by autoradiography. (A) Enzymatic deglycosylation analysis of ³⁵S-labeled immune complexes. (B) Enzymatic deglycosylation analysis of immune complexes derived from ³H-glucosamine labeled cells. (C) Acylation analysis of immune complexes from ³H-palmitate labeled cells.