Effects of siRNA interference on the level of SLC34A2 mRNA and MX35 protein expression in SK-RC-18 cells. Cells were transfected with SLC34A2 siRNA or control siRNA (NT1 and NT2) in the presence of Lipofectamine 2000. Cells were assayed 72 hours after transfection. (A) Total RNA was extracted and SLC34A2 mRNA levels were determined by real-time RT-PCR. (B) Cells were lysed and the level of protein expression was analyzed by SDS-PAGE and Western blotting using mAb MX35 and an anti-actin antibody.