Induction of ADCC by the sera of immunized mice. ADCC assay using MCF-7 (A) and KB (B) cells as target. Three groups of Swiss mice were immunized with PBS, BTAA alone, or BTAA combined with NLP weekly for 4 weeks. Seven days after the last injection, sera from individual mice were collected and tested in ADCC assays. Human peripheral blood mononuclear cells were used as effector cells and tumor cells (MCF-7 and KB) were used as target. (C) ADCC assay using MCF-7 as target and sera obtained by immunization through different routes. Swiss mice were immunized with BTAA and NLP through s.c., i.v. or i.p. routes weekly for 4 weeks. Seven days after the last injection, sera from individual mice were collected and tested in ADCC assays. Data obtained by using an E:T ratio of 10:1 and immune sera diluted 1:100 is presented. Each data represents the mean value ± 1 SD. *Significantly higher as compared to other values (P < 0.001).