Determination of apparent steady-state dissociation constants for the interaction of anti-FRα antibodies with human folate receptor-alpha. (A) Rate constants and steady-state dissociation constants measured using immobilized, native FRα, and the indicated antibodies as analyte. For each experiment, 5 concentrations of antibody were assayed in triplicate. (B) Sensorgram from analysis of MORAb-003 binding kinetics to immobilized, native FRα. MORAb-003 concentrations are indicated next to the curves.