Immunofluorescence (A-C) and IHC (D-G) analysis of XAGE-1b. (A) Expression of XAGE-1b in 293T cells transfected with pcDNA3.1/XAGE-1b (green) by flow cytometry. Solid line, empty vector. (B) Fluorescence microscopy analysis. (C) Nuclei are shown stained with PI (red). XAGE-1b derived fluorescence is merged with the corresponding PI recordings. (D), (E), (F) and (G) are IHC analysis of testis, lung cancer, hepatocellular carcinoma and gastric cancer, respectively. Magnification 400x.