Generation of a mAb against XAGE-1b. (A) Reactivity of USO9-13 mAb with recombinant proteins determined by ELISA. (B) Immunoblotting of lysate of 293T cells transiently transfected with pcDNA3.1/XAGE-1b using USO9-13 mAb as probe. Mock, empty vector. (C) Reactivity of USO9-13 mAb (1 µg/ml) with 25-mer overlapping peptides from XAGE-1b determined by ELISA. (D) Reactivity of USO9-13 mAb (10 µg/ml) against C-terminal peptides from XAGE-1b-related molecules. Shaded, conserved residues.