ELISPOT assay demonstrating the dose-dependent IFN-γ secretion by HPV 16 E6 52-61-specific T cell clone cells upon stimulation by tumor cell line #1. The experiment was performed in triplicate wells. The wells contained 1 x 10⁵ EBV-LCL cells from patient #1, the T cell clone (10,000, 30,000, or 50,000 cells), and tumor cell line #1 cells (1,000, 3,000, 5,000, 10,000, or none).