Cancer Immunity, Vol. 6, p. 5 (9 March 2006) Submitted: 17 March 2005. Resubmitted: 21 December 2005. Accepted: 22 December 2005.
Kevin P. O'Boyle1, Susan Coatsworth2, Gillian Anthony2, Mark Ramirez2, Edward Greenwald2, Ronald Kaleya2, Jacob J. Steinberg2, Janice P. Dutcher1 and Peter H. Wiernik1
1Our Lady of Mercy Cancer Center, New York Medical College, 600 E. 233rd Street, Bronx, NY 10466, USA
2Montefiore Medical Center, Albert Einstein College of Medicine, 111 E. 210th Street, Bronx, NY 10467, USA
Communicated by: V Cerundolo
Resected carcinoma patients were immunized 3-5 times with ovine submaxillary gland mucin (OSM) containing predominantly sialylated Tn (sTn), completely desialylated ovine submaxillary gland mucin (dOSM) containing predominantly Tn, or 50% desialylated OSM containing Tn and sTn plus bacillus Calmette-Guerin (BCG) as an immunologic adjuvant. Pre- and postimmunization sera were quantified by ELISA, whole-cell ELISA, and immune stain dot blots. Fifteen of 17 patients produced IgG antibody titers from 40 to 5120 times more reactive with OSM and dOSM postimmunization. More importantly, these IgG antibodies reacted with LS-174T, a human colon carcinoma cell line. Significant DTH-like responses (1-17 cm) were observed in 15 of 17 patients; the strength of these responses was dependent on the presence or absence of sialic acid. Biopsies of these DTH-like reactions revealed infiltration with some CD8+ lymphocytes and mast cells. These results suggest that a single 9-carbon sugar can affect cellular immune responses to mucin antigens. It is thought that these large erythematous, nonindurated cellular reactions are antibody-mediated Arthus-like reactions. OSM, and especially dOSM, were also found to inhibit lymphocyte proliferation.
Copyright © 2006 by Kevin O’Boyle