Real-time PCR analysis of L514S expression in lung squamous tumors. A panel of 66 cDNA samples composed of 32 tumor specimens and 34 normal tissues was tested. L514S mRNA expression was analyzed by real-time PCR. Overexpression was defined as >10-fold expression as compared to the highest expression in normal tissue except for testis. L514S was overexpressed in 11 of 20 lung squamous tumors.