Expression of A34 mRNA transcripts in various normal and malignant human tissues. A34 mRNA expression was analyzed by conventional end-point RT-PCR analysis in normal adult tissues using a commercially available source of cDNA templates (Rapid-Scan™ Gene Expression Panels, Origene, Rockville, MD). Four different cDNA template concentrations were analyzed ranging from 0.001 ng to 1.0 ng. At cDNA concentrations of 1.0 ng and 0.1 ng, A34 transcripts were detected only in normal testis and stomach, but not in 20 other normal tissues. A34 transcripts were not detected at lower cDNA concentrations. Lane assignments: 1, brain; 2, heart; 3, kidney; 4, spleen; 5, liver; 6, colon; 7, lung; 8, small intestine; 9, muscle; 10, stomach; 11, testis; 12, placenta; 13, salivary gland; 14, thyroid; 15, adrenal gland; 16, pancreas; 17, ovary; 18, uterus; 19, prostate; 20, skin; 21, PBLs; 22, bone marrow.