Mouse endosialin RNA expression. Quantitative real-time RT-PCR of total RNA extracted from whole mouse embryos at stages E11.5, E13.5 and E14.5, and distinct newborn and adult organs. The relative expression of endosialin is normalized to the amount of mGAPDH in the same cDNA sample, the standard deviation (indicated by the error bars) is calculated from a set of 3 independent experiments. Note that whole-embryo endosialin mRNA levels increase from E11.5 to E14.5, and are markedly lower, in the selected organs, in newborn and adult animal. The newborn kidney and adult pregnant uterus show higher mRNA levels, consistent with the immunohistochemical staining results.