MAD-CT-1 and MAD-CT-2 are predominantly expressed in testis and are expressed in some prostate cancer tissues. (A) Gene-specific primers for MAD-CT-1, MAD-CT-2, or actin were used to detect transcript in cDNA samples obtained from normal human liver, lung, testis, colon, bladder, prostate, heart, brain, kidney, placenta, skeletal muscle, spleen or thymus (Biochain, Hayward, CA, USA). (B) Gene-specific primers were used to amplify MAD-CT-1, MAD-CT-2, or actin from cDNA specimens derived from metastatic prostate cancer tissue specimens (n = 10). A plus sign indicates a positive control using plasmid DNA encoding either MAD-CT-1 or MAD-CT-2 as template DNA. A minus sign indicates a negative control using nonspecific plasmid DNA as template DNA.