| When
the tumor stroma does not present antigen, gp33-Hi tumors escape
a CTL response as ALVs.
| Cell
line |
Stroma |
T
cells |
Tumor
outgrowth in experiment no.1 |
Total |
| 1 |
2 |
3 |
4 |
Tumor
growth |
Antigen
retained2 |
| gp33 |
C3H |
- |
- |
1/1 |
1/1 |
- |
2/2 |
2/23 |
| + |
1/2 |
4/4 |
- |
- |
5/6 |
0/54 |
| B6 |
- |
1/1 |
1/1 |
1/1 |
2/2 |
5/5 |
2/25 |
| + |
0/2 |
0/2 |
- |
0/3 |
0/7 |
N.A.6 |
| B6
→ C3H |
- |
- |
- |
- |
1/1 |
1/1 |
N.D. |
| + |
- |
- |
- |
5/5 |
5/5 |
0/57 |
| C3H
→ B6 |
- |
- |
- |
- |
1/1 |
1/1 |
1/1 |
| + |
- |
- |
- |
3/3 |
3/3 |
N.D. |
| B6 → B6 |
- |
- |
- |
- |
1/1 |
1/1 |
N.D. |
| + |
- |
- |
- |
0/3 |
0/3 |
N.A. |
1C3H Rag2(-/-) and C57BL/6 Rag1(-/-) were challenged
with 1-3 x 106 cancer cells. 14-16 days after tumor
challenge, mice were treated i.v. with in vitro activated
P14 T cells. Tumor outgrowth was assessed after 30 d.
2Unless otherwise noted, averages are from two independent experiments.
360.6 ± 17.7% of the reisolated cells expressed antigen above background levels. The average fold increase in antigen expression was 41.4 ± 8.3.
40.47 ± 0.1% of the reisolated cells expressed antigen above background levels. The average fold increase in antigen expression was 4.4 ± 3.1.
561.6 ± 16.4% of the reisolated cells expressed antigen above background levels. The average fold increase in antigen expression was 38.9 ± 7.8.
6N.A. = Not applicable.
70.2 ± 0.1% of the reisolated cells expressed antigen above the secondary staining. The average fold increase in antigen expression was 1.1 ± 0.1. Averages are from one experiment.
|
