| ALVs
escape a systemic CTL response in gp33-Lo tumors.
| Type
of challenge2 |
Cell
line |
T
cells |
Tumor
outgrowth in experiment no.1 |
Total |
|
1 |
23 |
3 |
4 |
53 |
6 |
Tumor
growth |
Antigen
retained4 |
|
Unilateral |
gp33-Lo |
- |
1/1 |
- |
- |
- |
- |
- |
1/1 |
N.D.5 |
| + |
1/1 |
2/2 |
1/1 |
1/1 |
- |
- |
5/5 |
N.D. |
|
gp33-Hi |
- |
1/1 |
- |
- |
- |
- |
- |
1/1 |
N.D. |
| + |
0/1 |
0/2 |
0/1 |
0/1 |
- |
- |
0/5 |
N.A.5 |
|
Bilateral |
gp33-Lo |
- |
- |
1/1 |
1/1 |
1/1 |
1/1 |
2/2 |
6/6 |
2/26 |
| gp33-Hi |
- |
1/1 |
1/1 |
1/1 |
1/1 |
2/2 |
6/6 |
N.D. |
| gp33-Lo |
+ |
- |
- |
4/4 |
- |
4/4 |
3/3 |
11/11 |
0/77 |
| gp33-Hi |
- |
- |
0/4 |
- |
0/4 |
0/3 |
0/11 |
N.A. |
1Mice bearing two week established tumors received approximately 107 adoptively transferred naive P14 T cells. Tumor outgrowth was assessed after 30 days.
2In unilateral challenges, 2C mice received a single challenge of either 106 gp33-Hi cells or 106 gp33-Lo cells. In bilateral challenges, 2C mice received a challenge of 106 gp33-Lo cells on the left flank and a challenge of 106 gp33-Hi cells on the right flank.
3In experiment 2 and 5, CD8-purified P14 T cells were
transferred into 2C x Rag2(-/-) mice indicating that the rejection
of gp33-Hi tumors is not due to endogenous CD4+ T cells or B cells
in the 2C host.
4The averages of each group are from two independent experiments.
5N.D. = Not done. N.A. = Not applicable.
64OH-TAM treatment induced 67.0 ± 0.6% of the cells above background. The average fold increase above the untreated control was 19.7 ± 10.2.
74OH-TAM treatment induced 4.39 ± 2.1% of the cells above background. The average fold increase above the untreated control was 2.2 ± 0.8.
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