| Relationship
between antigen-specific T cells in peripheral blood (14 days
after vaccination) and at vaccination sites (2 days after vaccination)a.
| Patient
|
Peptide
|
ESO1157-167
|
ESO1157-165
|
ESO1155-163
|
Tyrosinase
|
MelanA/MART-1
analogue
|
Influenza
|
SLLMWITQCFL
|
SLLMWITQC
|
QLSLLMWIT
|
YMDGTMSQV
|
ELAGIGILTV
|
GILGFVFTL
|
| PB
|
VIL
|
PB
|
VIL
|
PB
|
VIL
|
PB
|
VIL
|
PB
|
VIL
|
PB
|
VIL
|
| 1 |
145/23
|
98/5
|
34/7
|
94/9
|
0
|
0
|
26/5
|
0
|
30/9
|
0
|
64/4
|
44/3
|
| 2 |
2
|
4
|
0
|
0
|
4
|
0
|
3
|
0
|
7
|
2
|
20
|
0
|
| 3 |
4
|
7
|
7
|
11
|
0
|
3
|
0
|
3
|
61/12
|
7
|
111/20
|
33/12
|
| 4 |
1
|
12
|
0
|
2
|
0
|
0
|
0
|
13
|
26/4
|
0
|
19
|
28/5
|
| 5 |
0
|
1
|
0
|
0
|
1
|
1
|
0
|
2
|
82/4
|
0
|
35/10
|
0
|
| 6 |
0
|
0
|
0
|
1
|
0
|
1
|
0
|
0
|
0
|
2
|
72/37
|
2
|
| 7 |
0
|
4
|
0
|
0
|
0
|
0
|
4
|
0
|
58/4
|
0
|
180/10
|
165/4
|
| 8 |
65/5
|
230/30
|
Nt
|
Nt
|
Nt
|
0
|
Nt
|
0
|
Nt
|
0
|
260/20
|
250/0
|
| 9 |
95/5
|
188/8
|
Nt
|
0
|
Nt
|
2
|
0
|
0
|
0
|
0
|
155/5
|
0
|
| 10 |
0
|
8
|
0
|
0
|
0
|
0
|
0
|
0
|
16
|
0
|
170/10
|
30/2
|
| Positive |
3/10
|
3/10
|
0/8
|
1/9
|
0/8
|
0/10
|
0/9
|
0/10
|
3/9
|
0/10
|
6/10
|
3/10
|
aA
positive reaction was scored when more than 40 spots were counted.
Shaded areas show positive reactions. The number of spots/background
is shown for all positive samples. Abbreviation: PB, peripheral
blood; VIL, vaccine infiltrating lymphocytes; Nt, not tested.
|
