TCR vbeta16 usage of NY-ESO-1_157-165-specific T cells in VILs and PBLs. In this assay, T cells were assessed by triple staining, CyChrome-conjugated anti-CD8 Ab, FITC-conjugated vbeta16 Ab, and PE-conjugated tetramer (NY-ESO-1_157-165-specific). (A), (B), and (C) correspond to a T cell line derived from PBLs. (D), (E), and (F) correspond to a T cell line derived from VILs. The gated populations represent cells which are both vbeta16 and tetramer positive.