Immunoblot analysis to detect anti-PSMA antibodies in patients’ sera at different stages after the onset of vaccination. LNCaP lysate was run on SDS-PAGE, transferred onto PVDF membrane, and treated with (A) serum from patient no. 1 and (B) serum from patient no. 3. Lane 1, serum sample before the start of vaccination; lane 2, serum sample at 3 mo after starting vaccination; lane 3, serum sample at 6 mo; lane 4, serum sample at 9 mo; lane 5, serum at 12 mo; lane 6, serum at 15 mo; lane 7, serum at 18 mo; lane 8, serum at 21 mo; lane 9, serum at 24 mo after the start of vaccination.