Immunoblot analysis of the reactions of sera from patients vaccinated with gene-based PSMA vaccine against LNCaP lysate. (A) Mr, molecular weight markers; lane 1, LNCaP lysate; lane 2, recombinant PSMA. The blots were incubated with monospecific anti-PSMA serum and subsequently with antirabbit IgG labeled with alkaline phosphatase (AP). (B) Mr, molecular weight markers; lanes 1-10, LNCaP lysates treated with sera from different patients vaccinated with gene-based PSMA vaccine and subsequently incubated with antihuman IgG labeled with AP.