Secretion of (A) TNF-alpha, (B) IL-12, and (C) IL-10 by monocytes treated with different agents after coculture, before being restimulated with tumor cells (protocol 2). Cytokine release by tumor cell-stimulated control monocytes was 3225 ± 1200 pg/ml, 1442 ± 1140 pg/ml, and 1356 ± 908 pg/ml for TNF-alpha, IL-12 (p40), and IL-10, respectively. Cytokine release by monocytes that were cocultured and restimulated with tumor cells (medium) was 1943 ± 763 pg/ml, 595 ± 642 pg/ml, and 2875 ± 1621 pg/ml for TNF-alpha, IL-12 (p40), and IL-10, respectively. The results are expressed as the percentage of control monocytes stimulated with tumor cells. The means from 5-6 independent experiments are shown, with error bars corresponding to 1 SD.