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Table 3

MHC class I and HLA-A2 expression in tumor cell lines.

Cell line

HLA-A2 typing

MHC class I expression a

HLA-A2 expression b

Isotype control

- IFN-gamma

+ IFN-gamma

Isotype control

- IFN-gamma

+ IFN-gamma

SK-ES-1

+

5

20

325

2

6

134

SK-LMS-1

+

7

184

543

6

107

551

SK-UT-1

+

6

263

412

4

5

8

SW 872

+

6

84

121

5

187

630

Saos-2

+

6

118

150

4

84

150

HOS

+

11

183

230

7

244

558

MES-SA

-

6

28

245

3

5

6

HT-1080

-

5

36

330

4

8

8

SK-MEL-37

+

4

78

158

4

38

63

Mel 275

+

5

76

* c

4

75

* c

aMHC-class-I expression was assessed by staining with mAb W6/32. The mean fluorescence intensity is reported.
bHLA-A2 expression was assessed by staining with mAb BB7.2. The mean fluorescence intensity is reported.
cCells died upon treatment with IFN-gamma.

 

Copyright © 2004 by Danila Valmori