|
Comparison
of NY-ESO-1 expression in sarcoma tumors as assessed by PCR or
IHC.
|
Histological subtype
|
Case no.
|
NY-ESO-1
|
|
PCR a
|
IHC b
|
|
Gastrointestinal stromal tumor
|
1
|
+++
|
3/pos
|
|
2
|
-
|
0/neg
|
|
3
|
+
|
2/pos
|
|
4
|
-
|
0/neg
|
|
5
|
-
|
0/neg
|
|
Synovial sarcoma
|
1
|
+++
|
2-3/pos
|
|
2
|
+++
|
3/pos
|
|
Uterine leiomyosarcoma
|
1
|
+
|
1/neg
|
|
2
|
-
|
1/neg
|
|
3
|
-
|
0/neg
|
|
4
|
+
|
2/pos
|
|
5
|
+++
|
3/pos
|
|
Leiomyosarcoma
|
1
|
-
|
0/neg
|
|
2
|
-
|
1/neg
|
|
3
|
-
|
0/neg
|
|
4
|
-
|
1/neg
|
|
5
|
-
|
0/neg
|
|
6
|
-
|
0/neg
|
|
7
|
-
|
1/neg
|
|
Angiosarcoma
|
1
|
+
|
1/neg
|
|
2
|
++
|
2/pos
|
|
3
|
-
|
1/neg
|
|
Malignant fibrous histiocytoma
|
1
|
++
|
2/pos
|
|
2
|
-
|
1/neg
|
|
3
|
-
|
1/neg
|
|
4
|
-
|
1/neg
|
|
5
|
-
|
0/neg
|
|
Liposarcoma
|
1
|
-
|
1/neg
|
|
2
|
+++
|
3/pos
|
|
3
|
-
|
0/neg
|
|
4
|
-
|
0/neg
|
|
5
|
-
|
0/neg
|
|
6
|
-
|
nd
|
|
Osteosarcoma
|
1
|
-
|
0/neg
|
|
Chondrosarcoma
|
1
|
+
|
0/neg
|
|
2
|
+
|
1/neg
|
aNY-ESO-1 expression at the RNA level was assessed
by semiquantitative RT-PCR.
bNY-ESO-1 expression at the protein level was assessed
by IHC using monoclonal antibody ES121 to NY-ESO-1. Scoring: 0,
no positive cells; 1, rare and scattered positive cells; 2, <
or = 50% positive cells; 3, >50% positive cells; nd, not done.
Interpretation: pos, positive; neg, negative.
|
