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Semiquantitative
RT-PCR analysis of apoptosis regulators. (A) Graphic analysis
of semiquantitative RT-PCR for Bcl2, Bcl7C, and Bax RNA levels
after 48 hr of treatment with either 2 µM IL-10 RNAi (hatched
bars) or 2 µM dsScr (gray bars) when compared to an untreated
control. Numbers represent percent of control values from triplicate
gels. Error bars are SEM. Asterisks indicate P < 0.05
for a Students t-test comparing dsScr to RNAi. (B) Representative
PCR gels stained with ethidium bromide. Lane 1 is control, lane
2 is 2 µM dsScr and lane 3 is 2 µM IL-10 RNAi.
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