Semiquantitative RT-PCR analysis of IL-10 and critical cell cycle components. (A) Graphic analysis of semiquantitative RT-PCR for IL-10, cdc25C, p27, IL-10 R1, IL-10 R2 and TNF-alpha RNA levels after 48 hours of treatment with either 2 µM IL-10 RNAi (hatched bars), 2 µM dsSCR (gray bars) as compared to an untreated control. Numbers correspond to the percentage of control values from triplicate gels and error bars to the SEM. Asterisks indicate P < 0.05 for a Student's t-test comparing dsScr to RNAi. (B) Representative PCR gels stained with ethidium bromide: Control (lane 1), 2 µM dsSCR (lane 2) and 2 µM IL-10 RNAi (lane 3).