IL-10 message analysis by real-time RT-PCR. Fluorescence of Taqman™ reporter dyes for IL-10 and ribosomal 18S are labeled. Black curves represent results from LNC cells treated for 72 hr with 2 µM IL-10 RNAi. Gray curves are untreated control. Ribosomal 18S was used to normalize samples. The graph is representative of three real-time PCR reactions.