IL-10 protein analysis by ELISA. The amount of IL-10 protein in LNC supernatants was measured by ELISA. Columns correspond to groups treated for 72 hr with either 2 µM IL-10 RNAi (hatched bar), 2 µM dsScr (black bar), or untreated control (gray bar). Error bars are the SEM of triplicate results. Asterisks indicate P < 0.05 for a Student's t-test comparing dsScr to RNAi.