RNAi modeling and annealing. (A) An idealized model of RNA folding for IL-10 based on enthalpy and free-energy calculations. Brackets indicate the location of the mouse IL-10 RNAi employed in these experiments. The sequence begins at nucleotide 317 and appears exposed in a hairpin. (B) Acrylamide gel of RNA stained with ethidium bromide. Lane 1 is dH₂O as a negative control, lane 2 is dsRNAi, lane 3 is ssRNAi strand 1, and lane 4 is ssRNAi strand 2. The size of the ladder on the far left (y-axis) is given in nucleotides. The arrow indicates the band for dsRNAi.