Quantification of clone AU-HN-15 mRNA in normal and tumor tissues by qRT-PCR. Messenger RNA copy number is expressed as the number of AU-HN-15 molecules in 100 ng of total RNA per 10⁵ molecules of beta-glucoronidase. (A) Normal tissues: (1) brain, (2) testis-1, (3) colon, (4) liver, (5) testis-2, (6) kidney-1, (7) lung, (8) trachea, (9) small intestine, (10) stomach, (11) thymus, (12) heart, (13), spleen, (14) bone marrow, (15) kidney-2. (B) HNSCC tumor tissues (1-11) and HNSCC cell lines (12-15): SCC-25 (12), HTB-4B (13), SCC-4 (14), CCL-30 (15). (C) Paired samples: HNSCC (1, 3, 5) and adjacent mucosae (2, 4, 6) from three primary tumors; HNSCC metastatic to lymph node (7) and paired normal tissue (8).