Activation of Melan-A-specific T cells. Forty-three of 49 patients were evaluable for immune responses and are listed according to their treatment groups. Melan-A specific responses were assessed by flow cytometry, IFN-gamma ELISPOT assays, and DTH. The numbers indicate the percentages of positive cells in CD8+ PBLs, except for columns 6-9 which show the percentages of cells negative for CD45RA and CD28 among multimer positive cells. PBLs were withdrawn before vaccination, after the first cycle (1^st cyc), and after additional ("continued") vaccination (cont v). Values in colored fields correspond to an increase of more than two-fold or of more than 20% in CD45RA- or CD28- antigen-specific T cells, respectively (comparing values after versus before vaccination). Columns 5, 7, and 9 show the results of the blood sample with the highest value among PBLs withdrawn at various time points after vaccination. Fields are left empty if no additional vaccination cycle was completed (columns 5 and 12), if percentages of multimer positive cells were too low for phenotyping for CD45RA and CD28 (columns 6-9), or if DTH was not performed (columns 13 and 14). Abbreviations: Cyc, cycle; na, not applicable; ni, not interpretable.