Quantitation of Melan-A-specific T-cell activation by immune scoring. No activation was found in 6 patients vaccinated with peptides in saline or in 8 patients vaccinated with peptides in adjuvant (AS02B or IFA) and IL-2. One patient responded to peptides in AS02B, and 12 patients responded to peptides in IFA. Melan-A-specific T cells were assessed by flow cytometry and IFN-gamma ELISPOT assay, and immune scores were calculated as described in Materials and Methods.