Vaccination schedule. Vaccinations were given in cycles; each cycle consisted of four injections (vertical lines). Up to four additional and similar cycles (continued vaccination, not shown) were given to patients without major disease progression, with 2-mo intervals between cycles. Vaccines contained 100 µg each of Melan-A and influenza peptides injected as mixtures in saline or together with 660 µl AS02B or 600 µl IFA. Before starting treatment and 2 wk after the last vaccine of each cycle, peripheral blood cells (PBLs) were withdrawn (vertical arrows) for lymphocyte analyses by flow cytometry and IFN-gamma ELISPOT assays. The patients in treatment group IIIb were on a different schedule (not shown) since vaccines were given biweekly and PBLs were obtained 1 wk after the last vaccine of each cycle.