Gp96/MART-1 complexes reconstituted at 50°C and 4°C. SDS-PAGE of the complexes stained by Coomassie shows no differences in homogeneity irrespective of the complexing temperature. The autoradiography from this SDS gel illustrates the differences in complexing efficiency: At 4°C, 0.16 ng peptides were complexed to 1 µg of gp96, whereas at 50°C, 1.7 ng peptides were complexed to 1 µg of gp96.