Complex stability at different storage temperatures. (A) The complexes proved to be relatively stable at -20°C. Only about 5% of the complexed peptides dissociated within a 24-h period. The amount of peptides dissociating increased with increasing temperature. After 24 h at 20°C, almost 30% of peptides dissociated, while at the physiological temperature of about 37°C more than 60% of the complexed peptides dissociated. (B) The peptide dissociation at 37°C was analyzed in more detail. After 1 h, only 2% of the peptides were dissociated, whereas the amount of peptides dissociated increased to 25% after 6 h and 60% after 18 h.