Recognition of naturally processed NY-ESO-1. (a) A panel of CD4+ T cell clones were tested in ELISPOT assays against autologous DCs pulsed with recombinant NY-ESO-1 protein (black bars) or irrelevant recombinant SSX protein (white bars), or infected with Ad2/ESO (horizontal stripes), v.v. ESO (checkered bars), or v.v. WT (gray bars). (b) CD4+ T cell clone NW 1662-CD4-2 was tested for specific recognition of autologous DCs pulsed with lysates prepared from NY-ESO-1-positive (NW-MEL-38 and SK-MEL-37) or NY-ESO-1-negative (NW-MEL-8) melanoma cell lines in ELISPOT assays.