|
Titration
of the capture and detection Abs for use in IL-5 ELISPOT assays.
|
Detection
Ab
(µg/ml)
|
Spots/50,000 CD4+ cells plated per well (control)
|
|
Capture Ab (µg/ml)
|
|
0.63
|
1.25
|
2.5
|
5
|
|
0.25
|
7 (1)
|
47 (22)
|
344 (30)
|
307 (21)
|
|
0.5
|
7 (2)
|
222 (24)
|
397 (31)
|
466 (25)
|
|
1
|
117 (19)
|
256 (19)
|
384 (43)
|
357 (35)
|
|
2
|
42 (16)
|
183 (7)
|
270 (34)
|
373 (21)
|
|
