Specificity controls of CD4+ T cell presensitization from patient 1 in IL-5 ELISPOT assays. CD4+ T cells were presensitized with the indicated peptides and tested by IL-5 ELISPOT at d25 against autologous T cell APCs pulsed with peptides NP 206-229 or ESO 157-170. The results represent the mean number of spots per 50,000 effector CD4+ T cells in duplicate wells.