|
Genes
|
Primers
|
PCR
Conditions
|
No.
of Cycles
|
Product
Size (bp)
|
|
NY-ESO-1
|
5'-CAGGGCTGAATGGATGCTGCAGA-3'
5'-GCGCCTCTGCCCTGAGGGAGG-3'
|
94°C,
1 min
60°C, 1 min
72°C, 1 min
|
35
|
332
|
|
LAGE-1
|
5'-CTGCGCAGGATGGAAGGTGCCCC-3'
5'-GCGCCTCTGCCCTGAGGGAGC-3'
|
94°C,
1 min
60°C, 1 min
72°C, 1 min
|
35
|
332
|
|
MAGE-1
|
5'-CGGCCGAAGGAACCTGACCCAG-3'
5'-GCTGGAACCCTCACTGGGTTGCC-3'
|
94°C,
1 min
72°C, 1 min
72°C, 2 min
|
35
|
421
|
|
MAGE-3
|
5'-GAAGCCGGCCCAGGCCCAGGCTCG-3'
5'-GGACGATTATCAGGAGGCCTGC-3'
|
94°C,
1 min
66°C, 1 min
72°C, 2 min
|
35
|
778
|
|
MAGE-4
|
5'-GAGCAGACAGGCCAACCG-3'
5'-AAGGACTCTGCGTCAGGC-3'
|
94°C,
30 s
68°C, 30 s
72°C, 30 s
|
35
|
446
|
|
SCP-1/
HOM-TES-14
|
5'-GTACAGCAGAAAGCAAGCAACTGAATG-3'
5'-GAAGGAACTGCTTTAGAATCCAATTTCC-3'
|
94°C,
1 min
54°C, 1 min
72°C, 1 min
|
40
|
565
|
|
SSX-1
|
5'-CTAAAGCATCAGAGAAGAGAAGC-3'
5'-AGATCTCTTATTAATCTTCTCAGAAA-3'
|
94°C,
1 min
56°C, 1 min
72°C, 1 min
|
40
|
422
|
|
SSX-2/
HOM-MEL-40
|
5'-GTGCTCAAATACCAGAGAAGATC-3'
5'-TTTTGGGTCCAGATCTCTCGTG-3'
|
94°C,
1 min
67°C, 1 min
72°C, 1 min
|
35
|
435
|
|
SSX-4
|
5'-AAATCGTCTATGTGTATATGAAGCT-3
5'-GGGTCGCTGATCTCTTCATAA-3'
|
94°C,
1 min
60°C, 1 min
72°C, 1 min
|
35
|
415
|
|
CT7/
MAGE-C1
|
5'-GACGAGGATCGTCTCAGGTCAGC-3'
5'-ACATCCTCACCCTCAGGAGGG-3'
|
94°C,
1 min
60°C, 1 min
72°C, 1 min
|
35
|
632
|
|
CT10/
MAGE-C2/
HCA587
|
5'-TGTACTGCGCTGCCGTGAGACTGGTG-3'
5'-AAGCTCCATGAACTCACGGGCTCTCTTG-3'
|
94°C,
1 min
68°C, 1 min
72°C, 1 min
|
35
|
752
|
|
G3PDH
(control)
|
5'-GTCAACGGATTTGGTCGTATT-3'
5'-AGTCTTCTGGGTGGCAGTGAT-3'
|
94°C,
1 min
56°C, 1 min
72°C, 1 min
|
24
|
540
|
