NY-ESO-1 expression detected by RT-PCR in gastric cancer specimens. Twelve of 101 gastric cancer samples yielded the amplified band corresponding to NY-ESO-1. No tumor-adjacent tissue yielded the band. Lanes: M, molecular standard; 1-12, NY-ESO-1 positive samples. G3PDH controls: Ca, gastric cancer tissue samples; Adj, cancer-adjacent tissue samples.