|
Normal
tissue expressiona profiles of CT genes as determined
by RT-PCRb.
|
CT Genes with Testis-Restricted mRNA Expression (µg cDNA product)
|
|
BAGE/CT2.1 (0.02), MAGEE1/ CT10 (>2.0), CSAGE/CT24.1 (0.01), SPO11/CT35 (0.02), MAGEB1/CT3.1 (>2.0), SAGE/CT14 (0.02), MMA-1/CT25.1a (0.02), TPX1/CT36 (0.02), GAGE1/CT4.1 (>2.0), LIP1/CT17 (0.02), HOM-TES-85/CT28 (0.75), FTHL17/CT38 (>2.0), SSX2/CT5.2 (0.04), NA88/CT18 (0.01), LDHC/CT32 (0.04), TPTE/CT44 (0.08), BRDT/CT9 (0.02), CTAGE-1/CT21.1 (0.02), MORC/CT33 (0.06)
|
|
CT Transcripts Detected in 2 or Fewer Non-gametogeic Tissues
|
|
CT Transcript
|
Tissue (µg cDNA product)
|
|
MAGEA1/CT1.1
|
testis (>2.0), pancreas (0.02)
|
|
NY-ESO-1/CT6.1
|
testis (>2.0), placenta (0.05), pancreas (0.02), liver (0.11)
|
|
MAGEC1/CT7.1
|
testis (0.14), pancreas (0.09)
|
|
SYCP1/CT8
|
testis (>2.0), pancreas (0.03)
|
|
SPANXC/CT11.3
|
testis (2.0), placenta (0.10), pancreas (1.2), liver (0.19)
|
|
HAGE/CT13
|
testis (0.02), pancreas (0.01)
|
|
ADAM2/CT15
|
testis (0.03), pancreas (0.02)
|
|
SGY-1/CT34
|
testis (1.87), ovary (0.01), pancreas (0.03), spleen (0.01)
|
|
NY-SAR-35/CT37
|
testis (1.3), placenta (0.01), pancreas (0.02), liver (0.01)
|
|
TDRD1/CT41.1
|
testis (0.08), pancreas (0.02), spleen (0.01)
|
|
Differentially Expressedc CT Genes
|
|
CT Transcript
|
Tissue (µg cDNA product)
|
|
XAGE-1a/CT12.1a
|
testis (>2.0), placenta (0.03), pancreas (0.02), spleen (0.02), thymus (0.02), lung (>2.0), skeletal muscle (0.01)
|
|
PAGE-5/CT16.1
|
testis (0.07), pancreas (0.01), spleen (0.01), kidney (0.02), liver (0.02)
|
|
CAGE/CT26
|
testis (0.23), pancreas (0.03), leukocytes (0.01), brain (0.01)
|
|
BORIS/CT27
|
testis (>2.0), pancreas (0.03), prostate (0.01), thymus (0.01), kidney (0.02)
|
|
D40/CT29
|
testis (0.05), placenta (0.02), pancreas (0.01), thymus (0.02), colon (0.01), lung (0.01)
|
|
NXF2/CT39
|
testis (>2.0), pancreas (0.02), prostate (0.02), brain (0.02), kidney (0.02), lung (0.03)
|
|
TEX15/CT42
|
testis (>2.0), ovary (0.02), pancreas (0.07), spleen (0.02), liver (0.06), small intestine (0.06), prostate (0.01)
|
|
FATE/CT43
|
testis (>2.0), ovary (0.01), thymus (0.06), heart (0.02), kidney (0.02), lung (0.02), skeletal muscle (0.02), pancreas (0.02)
|
|
TAF7L/CT40
|
testis (0.15), placenta (0.03), pancreas (0.03), prostate (0.01), brain (0.01), kidney (0.03), liver (0.03), small intestine (0.02)
|
|
Ubiquitously Expressed CT Genes (Figure 1)
|
|
IL-13r alpha/CT19, SPA17/CT22, PLU-1/CT31, TSP50/CT20, OY-TES-1/CT23
|
aCommercially
available, normalized cDNA (MTC panels, BD Biosciences, Palo Alto
CA) derived from 13 non-gametogenic tissues (colon, leukocyte,
prostate, spleen, thymus, brain, heart, kidney, liver, lung, skeletal
muscle, pancreas and small intestine), as well as testis, ovary
and placenta.
b35 cycles of PCR amplification were performed using
primer sequences and annealing temperatures described in primary
publications.
cDifferentially Expressed CT Transcripts were detected
in 3-6 of the 13 non-gametogenic tissues analyzed.
|
