Verification of CT expression in normal pancreas by RT-PCR. End-point RT-PCR assay was used to verify the mRNA expression of 25 CT transcripts in a second sample of pancreatic cDNA (see text). The presence of mRNA encoding MAGEA1/CT1.1, XAGE-1a/CT12.1a, HAGE/CT13, PAGE-5/CT16A, IL13RA/CT19, TSP50/CT20, SPA17/CT22, OY-TES-1/CT23, BORIS/CT27, D40/CT29, PLU-1/CT31, SGY-1/CT34, NY-SAR-35/CT37, NXF2/CT39, TAF7L/CT40, and TDRD1/CT41.1 was confirmed in normal pancreas. Lane assignments are as follows: 1, OY-TES-1/CT23; 2, MAGEC1/CT7.1; 3, NY-ESO-1/CT6.1; 4, SYCP1/CT8; 5, BORIS/CT27; 6, ADAM2/CT15; 7, IL13RA/CT19; 8, PLU-1/CT31; 9, TDRD1/CT41.1; 10, TEX15/CT42; 11, MAGEA1/CT1.1; 12, TSP50/CT20; 13, NXF2/CT39; 14, SGY-1/CT34; 15, CAGE/CT26; 16, TAF7L/CT42; 17, D40/CT29; 18, PAGE-5/CT16.1; 19, NY-SAR-35/CT37; 20, SPA17/CT22; 21, HAGE/CT13; 22, SAGE/CT14; 23, SPANXC/CT11.3; 24, XAGE-1a/CT12.1a; 25, FATE/CT43.