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Table 2

NY-ESO-1 typing and monitoring.

Standardized Assays

Antigen expression

RT-PCR, immunohistochemistry (IHC)

Antibody

ELISA, Western blot

CD8+ T cell

ELISPOT, tetramer, cytoxicity

DTH

Skin test: Peptide or protein

Assays under Development

CD4+ T cell

ELISPOT, tetramer, cytokine release

MHC expression

IHC

Peptide/MHC complex

mAb, IHC

In situ immunology

IHC

 

Copyright © 2003 by Lloyd J. Old