Analysis of the immune response in patient 1. (A) Brisk infiltration of tumor nodule by tumor infiltrating lymphocytes (TILs). (B) Staining for CD8. (C) CTL activity of isolated TILs, demonstrating specificity for Melan-A, and a Melan-A-positive HLA-A2+ cell line (ME 272). Histograms show E:T ratios of 60:1, 20:1, 7:1 and 2:1, respectively for each target: Melan-A_27-35 peptide, tyrosinase_368-376 peptide, tyrosinase_1-9 peptide, MAGE-3 A2_271-279 peptide, ME 272 cell line (Melan-A and HLA-A2 +ve), influenza_58-66 peptide, T2 cells alone and K562 cells.