|
Comparison
of different serological technologies for the detection of anti-NY-ESO-1
immune responsesa.
|
Sample No.
|
RAYSb
|
ELISAc
|
Western Blotd
|
Phage Displayd
|
|
FACS
|
Ratio
|
|
F 1
|
49.68
|
21.6
|
1284
|
+++
|
+
|
|
F 2
|
3.12
|
1.28
|
421
|
-
|
-
|
|
F 3
|
11.63
|
4.02
|
410
|
++
|
-
|
|
F 4
|
3.2
|
1.47
|
589
|
-
|
+
|
|
F 5
|
12.88
|
5.37
|
1064
|
++
|
+
|
|
F 6
|
57.11
|
16.22
|
1008
|
+++
|
+
|
|
F 7
|
2.78
|
1.22
|
201
|
-
|
-
|
|
F 8
|
2.97
|
1.28
|
260
|
-
|
-
|
|
F 9
|
2.89
|
1.23
|
316
|
-
|
-
|
|
F 10
|
9.49
|
4.12
|
884
|
+
|
+
|
|
F 11
|
6.12
|
2.18
|
742
|
+
|
+
|
|
F 12
|
11.45
|
3.78
|
1003
|
++
|
+
|
|
F 13
|
35.38
|
15.72
|
1537
|
++
|
+
|
|
F 14
|
3.3
|
2.64
|
658
|
+
|
-
|
|
F 15
|
5.57
|
2.71
|
808
|
+
|
-
|
|
F 16
|
5.13
|
2.26
|
534
|
+
|
+
|
|
F 17
|
28.84
|
13.54
|
1389
|
+++
|
+
|
|
F 18
|
8.03
|
2.84
|
812
|
++
|
+
|
|
F 19
|
6.65
|
3.69
|
1161
|
+
|
+
|
|
F 20
|
62.04
|
27.45
|
1037
|
+++
|
+
|
|
F 21
|
63.75
|
27.48
|
1014
|
+++
|
+
|
|
F 22
|
4.22
|
2.11
|
449
|
+
|
+
|
|
F 23
|
18.95
|
7.52
|
1122
|
+++
|
+
|
|
F 24
|
47.55
|
19.02
|
997
|
+++
|
+
|
|
F 25
|
13.67
|
5.69
|
1595
|
+++
|
+
|
|
F 26
|
31.23
|
13.17
|
1299
|
++
|
+
|
|
F 27
|
59.12
|
26.6
|
1340
|
+++
|
+
|
|
F 28
|
2.72
|
2.16
|
772
|
+
|
-
|
|
F 29
|
9.1
|
3.8
|
874
|
+
|
-
|
|
F 30
|
6.49
|
2.58
|
274
|
(+)
|
-
|
|
F 31
|
3
|
1.15
|
246
|
-
|
-
|
|
F 32
|
2.85
|
1.84
|
302
|
-
|
-
|
|
F 33
|
3
|
1.26
|
215
|
-
|
-
|
|
F 34
|
3
|
1.16
|
137
|
-
|
-
|
|
F 35
|
3.99
|
1.54
|
280
|
-
|
-
|
|
F 36
|
4.5
|
1.48
|
429
|
-
|
-
|
|
F 37
|
3.2
|
1.22
|
593
|
-
|
-
|
|
F 38
|
3.4
|
1.27
|
271
|
-
|
-
|
|
F 39
|
3.3
|
1.34
|
274
|
-
|
-
|
|
F 40
|
3.7
|
1.35
|
225
|
-
|
-
|
|
F 41
|
3
|
1.18
|
133
|
-
|
-
|
|
F 42
|
<0
|
1.52
|
312
|
-
|
-
|
|
F 43
|
4.1
|
1.45
|
194
|
-
|
-
|
|
F 44
|
3.1
|
0.8
|
453
|
-
|
-
|
|
F 45
|
3.4
|
1.31
|
224
|
-
|
-
|
|
F 46
|
3.4
|
1.29
|
346
|
-
|
-
|
|
F 47
|
3.4
|
1.27
|
352
|
-
|
-
|
|
F 48
|
2.2
|
1.6
|
360
|
-
|
-
|
|
F 49
|
3.1
|
1.18
|
287
|
-
|
-
|
|
F 50
|
3.5
|
0.34
|
199
|
-
|
-
|
aFifty serum samples from patients with different malignancies
were analyzed in a blind fashion. Serum samples were diluted 1:100
for RAYS and phage display, 1:400 for ELISA and 1:250 for Western
blot analysis. Individual values highlighted in bold are considered
positive.
bAntibody binding to yeast was determined by FACS and
the mean fluorescence intensity values are given. The RAYS ratio
was determined by dividing the fluorescence intensity obtained
by the individual serum sample on NY-ESO-1 protein positive yeast
by the fluorescence intensity that was obtained on yeast expressing
an irrelevant antigen. Samples with a ratio of two or more were
considered positive.
cThe mean ELISA values (OD at 405 nm) of triplicate
analyses are given.
dThe intensity of the signal detected by Western blotting
or phage analysis was determined visually.
|
