HLA-A*0201 restriction of SSX2-derived p103-111 stimulated CD8+ T-cells. (A) COS7/A2 cells were loaded with decreasing concentrations of SSX2 p103-111 peptide (black) ranging from 2 to 0.002 µg/ml. For blocking, the anti-pan human MHC-I antibody (clone W6/32) (grey) or the anti-HLA-A*02 antibody (clone BB7.2) (white) was added. (B) To displace SSX2 p103-111, T2 cells were loaded with a mix of SSX2 p103-111 peptide (0.02 µg/ml) and increasing concentrations of the HLA-A*0201 binding, CMV-derived p495-503 peptide. Reactions were measured by IFN-gamma ELISPOT assay.