HER2_16-30 augments HER2_63-71-specific CD8+ T cell induction in the presence of mGM-CSF. Peptides (10 µg), either individually or in combination, were administered s.c. on the back of BALB/c mice in the presence (+) or absence (-) of mGM-CSF (1 µg/shot). One week after the last immunization, splenic CD8+ T cells were prepared and an ELISPOT assay was performed to determine the number of IFN-gamma positive spots as described in Materials and Methods. Target cells were HER2_63-71 (solid bars) or control HER2_780-788 (open bars) pulsed with P1.HTR. The data shown are the mean ± SEM of three independent experiments.