IFN-gamma ELISPOTs produced by CD8 T cells from prostate cancer patients. Purified CD8 T cells were stimulated with autologous CD8-depleted PBLs infected with Ad2/ESO. Responding CD8 T cells were tested for IFN-gamma ELISPOTs in response to autologous B cells infected with vaccinia/NY-ESO-1 (v.v. ESO) recombinant virus or wild-type vaccinia virus (v.v. WT). (A) Target cell titration. The number of IFN-gamma ELISPOTs produced by 1x10⁵ CD8 T cells in response to 1x10⁵ or 5x10⁴ autologous B cells infected with either v.v. ESO (closed bar) or v.v. WT (open bar). (B) Effector cell titration. The number of IFN-gamma ELISPOTs produced by 1x10⁵, 5x10⁴, 2x10⁴ CD8 T cells against 1x10⁵ autologous B cells infected with either v.v. ESO (closed bar) or v.v. WT (open bar).