Expression of NY-ESO-1 mRNA in benign prostatic hyperplasia and prostate cancer. (A) Conventional RT-PCR analysis for NY-ESO-1 mRNA expression in BPH and prostate cancer: mRNA from testis (lane 1), BPH (lanes 2 and 3), stage C prostate cancer (lanes 4 and 5), stage D1 prostate cancer (lane 6), stage D2 prostate cancer (lanes 7 and 8), a bone metastatic lesion (lane 9) of the tumor in lane 8. (B) Quantitative real-time RT-PCR analysis for NY-ESO-1 mRNA expression in BPH and prostate cancer. Twenty (open square) and 33 prostate cancer specimens (open circle) which were positive and negative for NY-ESO-1 mRNA expression respectively by conventional RT-PCR and 8 BPH specimens (open diamond) were analyzed by real-time RT-PCR. The expression relative to testis (filled square) was determined as described.