Comparison of the efficiency of vaccination with tumor cells that secrete gp96 as compared to tumor cells that express gp96 on the cell surface. (A) Western blot analysis of gp96Ig expression in the total cell lysate of wild type Meth A (WT) and Meth A-96-Ig cells. (B) Tumor growth kinetics of WT Meth A in mice previously immunized with PBS, Meth A-96tm or Meth A-96-Ig cells. Each line represents the tumor growth kinetics for one mouse; data from two separate experiments were pooled. The number in the parenthesis indicates the number of tumor-free mice in each group at the end of the experiment.