|
Recognition
of Melan-A peptides and tumor cell lines by DR monospecific populations.
| |
Peptide recognitiona
|
Tumor recognitionb
|
MFIc
|
|
Melan-A27-35
|
Melan-A26-35
|
Melan-A26-35 A27L
|
NA8
|
NA8
+ P
|
Me 275
|
Me 275
+ P
|
Me 260
|
Me 260
+ P
|
Melan-A26-35 A27L
|
Tyrosinase368-376
|
|
Bulk DR
|
20
|
10
|
0.006
|
76
|
71
|
72
|
60
|
11
|
9
|
437
|
4
|
|
DR/A1B4
|
2.5
|
1
|
0.002
|
80
|
88
|
82
|
87
|
5
|
6
|
630
|
3
|
|
DR/A1B9
|
30
|
100
|
0.5
|
80
|
77
|
72
|
75
|
4
|
0
|
277
|
3
|
|
DR/A1D3
|
>1000
|
>1000
|
0.5
|
70
|
95
|
47
|
100
|
4
|
3
|
239
|
3
|
|
DR/A1E1
|
8
|
8
|
0.02
|
72
|
82
|
70
|
80
|
7
|
6
|
466
|
3
|
|
DR/A1E2
|
>1000
|
>1000
|
0.2
|
71
|
66
|
60
|
60
|
3
|
3
|
83
|
3
|
|
DR/A2B4
|
>1000
|
>1000
|
0.03
|
18
|
65
|
23
|
69
|
0
|
1
|
605
|
4
|
|
DR/A2B5
|
3
|
0.2
|
0.003
|
85
|
85
|
78
|
74
|
10
|
8
|
326
|
3
|
|
DR/A2D6
|
>1000
|
>1000
|
0.2
|
19
|
52
|
10
|
39
|
0
|
3
|
818
|
4
|
|
LAU 203/17
|
5
|
5
|
0.02
|
5
|
90
|
81
|
85
|
3
|
4
|
428
|
4
|
aPeptide concentration (nM) required for 50% maximal
lysis.
bSpecific lysis of NA8 (A2+ Melan-A-), Me 275 (A2+
Melan-A+), Me 260 (A2- Melan-A+) tumor cell lines at an E:T ratio
of 50:1.
cMean fluorescence intensity with A2/peptide multimers
incorporating the Melan-A26-35 A27L or Tyrosinase368-376
peptides.
|
